Western Transfer Buffer, 10X

NB001309100 Western Transfer Buffer, 10X - 100 ml
NB001309500 Western Transfer Buffer, 10X - 500 ml
NB001309102 Western Transfer Buffer, 10X - 1 L

10X Western Transfer Buffer Datasheet

10X Transfer Buffer is based Tris-Glycine and used as a transfer buffer during western blotting for the electro-transfer of proteins from SDS-PAGE gels to nitrocellulose or PVDF membranes.

- Ready to dilute – just use distilled deionized water for dilution

- Reduced preparation time – no reagents to weigh and filter

- Quality-controlled reagent – reproducible results

Preparation of 1x Western Transfer Buffer:

- For example, each 1L of 1x buffer, combine 100 ml of 10X Western Transfer Buffer, 200 ml of methanol and 700 ml ddH2O and store at 4°C for up to one week. Generally, 20% methanol is recommended, but it may be beneficial to decrease methanol concentration to 5-10% for increased transfer efficiency of large, low abundancy proteins.

- To avoid air bubbles in the buffer, prepare the buffer at least 10 minutes at room temperature before use.

Western Transfer Buffer, 10X

Western Transfer Buffer, 10X

Preparation of 1x Western Transfer Buffer:

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