Multi-color IR Western Blotting Pack

NB051202210 Multi-color IR Western Blotting Pack, Goat-anti-rabbit IgG IR700/ Goat-anti-mouse IgG IR800 - 10 assays

NB051202310 Multi-color IR Western Blotting Pack, Goat-anti-mouse IgG IR700/ Goat-anti-rabbit IgG IR800 - 10 assays

Multi-color IR Western Blotting Pack Datasheet

Multi-color IR Western Blotting Pack near infrared (IR) fluorescent Western blotting kits allow to two proteins at once, increasing the quality and quantity of information that can be gained from single blot. Assay a control alongside a protein of interest. Assay phosphorylated and un- phosphorylated isoforms of a protein simultaneously. The fluorescent dyes provided with the packs allow detection of low pg of protein. Multi-color IR Western Blotting Pack protocols save time and money since there is no need for disposable film, and the blot can be imaged immediately, without drying.

- MULTIPLEXING – Visualize two proteins simultaneously

- SENSITIVITY – Low picogram detection

- QUALITY REAGENTS – Includes Easy Protein-Free Blocking Buffer and Easy IR Western Wash Buffer

- FAST RESULTS – 3.5 hours for entire protocol

- FLUORESCENT DETECTION – Near IR (IR700 and IR800) kits with fluorescent secondary antibodies

- Principle of Multi-color Western Blotting

- Two colors, two proteins, simultaneously

By using dyes that have different spectral properties (excitation and emission), you can visualize them in different channels at the same time on a digital imager.

Also known as multiplexing, simultaneous detection with different antibodies is ideal for certain types of experiments. For example, assay a loading control alongside a protein of interest; assay for two proteins you want to quantify relative to each other; or, assay phosphorylated and non-phosphorylated isoforms of a protein simultaneously.

- Picogram detection levels and Linear data

Multi-color IR Western Blotting Pack

Multi-color IR Western Blotting Pack

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