Low Molecular Weight Protein Marker

NP041012610 - 0.5 mL
NP041012620 - 2.5 mL

Low Molecular Weight Protein Marker Manual

The Low Molecular Weight Protein Marker for SDS Electrophoresis is a liquid mixture of six purified proteins ranging from 14000 to 96000 Dalton when used in denaturing polyacrylamide, for use in molecular weight estimation in the presence of the detergent sodium dodecyl sulfate (SDS).

Thaw the marker at 37 °C for a few minutes.
Mix one part of LMW protein marker with one part of 2x sample buffer.
Heat this tube at 95 °C for 5 minutes to completely denature proteins.
Load the following volumes of the marker on SDS-PAGE:
- 10 μl per well for mini-gels;
- 15 μl per well for large gels.
If you want to store this sample buffer-diluted marker, chill on ice.
Make aliquots and keep them at –20 °C while not in use (additional heating is not required; for next uses, simply thaw the aliquots at 37-40 °C for a few minutes and mix until the solution is homogeneous and clear).

Low Molecular Weight Protein Marker

Low Molecular Weight Protein Marker

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