Acrylamide/Bis-acrylamide Solution

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Acrylamide Bis-acrylamide Solution Datasheet

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. But also acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel. Acrylamide/Bis-acrylamide solution has been used as a constituent in the preparation of stacking gel and resolving gel during sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel electrophoresis.

These acrylamide/bis-acrylamide solutions are available at the most common ratios for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide. Each batch of gel solutions is gel electrophoresis tested in our quality control laboratory.

General Gel Preparation:

- Mix acrylamide/bis solution, buffer and water in separate beakers.

- Deoxygenate the solutions briefly (1 to 3 minutes).

- Add to separating gel solution: 10% SDS solution (w/v in water), TEMED and APS solution (w/v 10% of APS), swirl gently to mix without incorporating air into the mixture.

- Casting the separating gel: fill slab gel sandwich with separating gel solution up to approx. two thirds. The remaining volume for the stacking gel should refer to a separation distance of 1.5-fold of the depth of the wells of the comb to be used. Overlay with isopropanol immediately (0.1 ml), let polymerize (approx. 15 - 20 min.).

- Add 10 % SDS in water to the stacking gel solution, then TEMED and 10 % ammonium persulfate solution (APS), swirl gently to mix without incorporating air into the mixture.

- Casting the stacking gel: fill up the slab gel sandwich to the top and let polymerize.

Applications:

Acrylamide: Bis-Acrylamide Ratio	% T	% C	Applications
19:1	30 / 40	5	DNA Sequencing / Peptide Separation
29:1	30 / 40	3.3	DNA Sequencing / Protein Separation
37.5:1	30 / 40	2.6	High Molecular Weight Protein Separation

For Orders:

ACRYLAMIDE/BIS-ACRYLAMIDE 30% SOLUTIONS
PRODUCT NO	PRODUCT NAME	PACK
NB001319050	Acrylamide/Bis-acrylamide 30% Solution (19:1)	50 mL
NB001319100	Acrylamide/Bis-acrylamide 30% Solution (19:1)	100 mL
NB001319250	Acrylamide/Bis-acrylamide 30% Solution (19:1)	250 mL
NB001319500	Acrylamide/Bis-acrylamide 30% Solution (19:1)	500 mL
NB001319102	Acrylamide/Bis-acrylamide 30% Solution (19:1)	1 L

NB001329050	Acrylamide/Bis-acrylamide 30% Solution (29:1)	50 mL
NB001329100	Acrylamide/Bis-acrylamide 30% Solution (29:1)	100 mL
NB001329250	Acrylamide/Bis-acrylamide 30% Solution (29:1)	250 mL
NB001329500	Acrylamide/Bis-acrylamide 30% Solution (29:1)	500 mL
NB001329102	Acrylamide/Bis-acrylamide 30% Solution (29:1)	1 L

NB001337050	Acrylamide/Bis-acrylamide 30% Solution (37.5:1)	50 mL
NB001337100	Acrylamide/Bis-acrylamide 30% Solution (37.5:1)	100 mL
NB001337250	Acrylamide/Bis-acrylamide 30% Solution (37.5:1)	250 mL
NB001337500	Acrylamide/Bis-acrylamide 30% Solution (37.5:1)	500 mL
NB001337102	Acrylamide/Bis-acrylamide 30% Solution (37.5:1)	1 L

ACRYLAMIDE/BIS-ACRYLAMIDE 40% SOLUTIONS
PRODUCT NO	PRODUCT NAME	PACK
NB001419050	Acrylamide/Bis-acrylamide 40% Solution (19:1)	50 mL
NB001419100	Acrylamide/Bis-acrylamide 40% Solution (19:1)	100 mL
NB001419250	Acrylamide/Bis-acrylamide 40% Solution (19:1)	250 mL
NB001419500	Acrylamide/Bis-acrylamide 40% Solution (19:1)	500 mL
NB001419102	Acrylamide/Bis-acrylamide 40% Solution (19:1)	1 L

NB001429050	Acrylamide/Bis-acrylamide 40% Solution (29:1)	50 mL
NB001429100	Acrylamide/Bis-acrylamide 40% Solution (29:1)	100 mL
NB001429250	Acrylamide/Bis-acrylamide 40% Solution (29:1)	250 mL
NB001429500	Acrylamide/Bis-acrylamide 40% Solution (29:1)	500 mL
NB001429102	Acrylamide/Bis-acrylamide 40% Solution (29:1)	1 L

NB001437050	Acrylamide/Bis-acrylamide 40% Solution (37.5:1)	50 mL
NB001437100	Acrylamide/Bis-acrylamide 40% Solution (37.5:1)	100 mL
NB001437250	Acrylamide/Bis-acrylamide 40% Solution (37.5:1)	250 mL
NB001437500	Acrylamide/Bis-acrylamide 40% Solution (37.5:1)	500 mL
NB001437102	Acrylamide/Bis-acrylamide 40% Solution (37.5:1)	1 L

Acrylamide/Bis-acrylamide Solution

Acrylamide/Bis-acrylamide Solution

ACRYLAMIDE/BIS-ACRYLAMIDE 30% SOLUTIONS

ACRYLAMIDE/BIS-ACRYLAMIDE 40% SOLUTIONS

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